Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES solution | 7365-45-9 | MFCD00006158 | 100 mL
HEPES solution | Mol Wt: 238.31 | 7365-45-9 | MFCD00006158 | 100 mL
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 titration25KG
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 100g
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 100g
Sigma Aldrich Fine Chemicals Biosciences PIPES Dipotassium Salt | 108321-27-3 | MFCD00069751 | 100g
PIPES Dipotassium Salt | Purity: 99% | Mol Wt: 378.55 | 108321-27-3 | MFCD00069751 | 100g
Sigma Aldrich Fine Chemicals Biosciences HEPBS >=99% (titration) | 161308-36-7 | MFCD00677124 | 25G
HEPBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 266.36 | 161308-36-7 | MFCD00677124 | 25G
Research Products International Corp PIPES FREE ACID ULTRAPURE, 1 KILOGRAM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
![Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92060-25.0.JPG-250.jpg)
Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams
- Substance Name: 3-(N-Morpholino)2-hydroxypropanesulfonic acid
- CAS Number: 68399-77-9
- Molecular Formula: C7H15NO5S
- Molecular Weight: 225.3
Dojindo Molecular Technologies Inc MES (100G), Good's buffer, CAS: 145224-94-8, pka=6.15, Active pH 5.5-7, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 100G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 100G
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 25g
BICINE | Purity: ≥99% (titration) | MW:163.17 | 150-25-4 | MFCD00004295 | 25g
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 25G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 25G
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra for molecul10G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Chem-Impex International, Inc. 3-N-Morpholinopropanesulfonic acid | 1132-61-2 | MFCD00006183 | 100G
3-N-Morpholinopropanesulfonic acid, 1132-61-2, MFCD00006183, 100G
Promocell GmbH PromoCell Hepes BSS (30 mM) 250 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).