Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, anhydrous | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid BioUltra, anhydrous | Purity: 99% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Teknova 1M MOPS Buffer, pH 7.4, 1000mL, Sterile. 2 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Teknova 10X TBS, pH 7.4. (247mM Tris, 1.37M NaCl) 1000mL, Sterile. 2 pack.
247mM Tris-HCl,1.37M Sodium Chloride
Teknova 20X TBS with 0.1% Tween-20. (400mM Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
3M Sodium Chloride, 400mM Tris-HCl
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
Research Products International Corp MOPS Free Acid 50 G
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes.
Heavy Metals: <5 ppm
pKa: (20°C) 7.20 ± 0.2
pH Range: 6.5-7.9
![Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32020-10000.0.JPG-250.jpg)
Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Research Products International Corp Tris-Glycine-SDS, 10 L
Common buffer used for running SDS-PAGE gels in electrophoresis.
1X Solution Contains:
TRIS/TRIS HCl 25 mM
Glycine 0.192 M
SDS 0.1%(w/v)
Research Products International Corp TBE, 4 L Cube
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Research Products International Corp RIPA Buffer 2X Solution, 250 ML
Enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins' immunoreactivity and biological activity.
Research Products International Corp Manganese Chloride 1M Solution, 100 ML
Manganese Chloride is used for dC and dG tailing of DNA.
Research Products International Corp Blot Stripping Buffer 4X Solution, 250 ML
Product Formulation: TRIS-HCl: 250 mM; SDS: 8%; pH: 6.8. Add mercaptoethanol according to protocol.
Instructions :
1. Dilute 4X Stripping Bufffer ( 1part Stripping Buffer: 3 parts distilled water) and add beta-mercaptoethanol (0.6%).
2. Wash the wet membrane in the stripping buffer for 5 minutes.
3. Incubate the membrane in stripping buffer for 30 minutes at 65 degrees Celius while agitating.
Research Products International Corp TAPS, 5 KG
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G